Journal: Vaccine

Article Title: An online decision tree for vaccine efficacy trial design during infectious disease epidemics: The InterVax-Tool

doi: 10.1016/j.vaccine.2019.06.019

Figure Lengend Snippet: Screenshot of InterVax-Tool at http://vaxeval.com . The decision tree with decisions and notes is presented on the top portion. The lower portion provides content on how relevant key considerations impact the active decision in the tree and provides the opportunity for users to take notes on each key consideration, building a description of the scenario during the process.

Article Snippet: We created a web-based interactive decision tree tool, InterVax-Tool, to support decision making on vaccine trial design.

Techniques:

Journal: Frontiers in Molecular Neuroscience

Article Title: MicroRNA-133b Negatively Regulates Zebrafish Single Mauthner-Cell Axon Regeneration through Targeting tppp3 in Vivo

doi: 10.3389/fnmol.2017.00375

Figure Lengend Snippet: miR-shRNA based gene silence of tppp3 diminishes regenerative length of M-cell (A) Diagram of miR-shRNA system. Sequence of ShRNA-5 was presented here, with the guide strand (bottom) highlighted in dark blue. (B) Quantitative RT-PCR analysis exhibited a deduction of tppp3 mRNA in shRNA-5 expressing embryos. (C) Confocal imaging of M-cell at 2 dpa. White asterisk: ablation point. Scale bar: 50 μm. (D) Regeneration length at 2 dpa. Student's two-tailed t -test, control vs. shRNA-1, P = 0.5807; control vs. shRNA-5, P = 0.0025. ** P < 0.01. Error bars represent S.E.M.

Article Snippet: Based on the Web-based shRNA design tool ( https://www.genscript.com ), five shRNAs (shRNA1-shRNA5) targeting the tppp3 gene were selected (Figure ). mCherry was used as a fluorescent reporter to mark the zebrafish embryos that expressed the miR-shRNA (Figure ).

Techniques: shRNA, Sequencing, Quantitative RT-PCR, Expressing, Imaging, Two Tailed Test